產(chǎn)品細(xì)節(jié)介紹
3BHB007211R102通道隔離模塊
波形捕捉
369在跟蹤緩沖區(qū)中每次發(fā)生跳閘時(shí)存儲(chǔ)16個(gè)A/D采樣周期。跟蹤內(nèi)存觸發(fā)器在S1中設(shè)置
首選項(xiàng)并確定存儲(chǔ)的行車前和行車后循環(huán)數(shù)。跟蹤緩沖區(qū)帶有時(shí)間和日期標(biāo)記并可能與事件記錄中的跳閘相關(guān)。當(dāng)發(fā)生跳閘時(shí)，以這種方式捕獲7個(gè)波形。這是3個(gè)相電流、接地電流和3個(gè)電壓波形。最后三條捕獲的記錄由369保留
信息存儲(chǔ)在易失性存儲(chǔ)器中，如果繼電器重新通電，信息將丟失。
要訪問捕獲的波形，請(qǐng)通過寫入跟蹤內(nèi)存緩沖區(qū)選擇器（地址30F5h），然后通過將其跟蹤存儲(chǔ)器通道（見下表）寫入跟蹤存儲(chǔ)器來選擇感興趣的波形頻道選擇器（地址30F6h）。然后從地址3100h到31FFh讀取跟蹤內(nèi)存數(shù)據(jù)。讀取的值為實(shí)際安培或伏特。套件存放如果打算在24小時(shí)。否則，檢測板、標(biāo)準(zhǔn)品、檢測試劑A、檢測試劑B應(yīng)儲(chǔ)存在-20°C下。避免重復(fù)凍融循環(huán)。將所有其他套件組件存放在4°C。這個(gè)基板不得凍結(jié)。打開單個(gè)試劑后建議在1個(gè)月內(nèi)使用該試劑盒。未使用的帶材板井應(yīng)儲(chǔ)存在-20°C的密封袋中干燥劑，以盡量減少接觸水分。不要使用套件超過有效期。
其他所需供應(yīng)品
? 帶450nm波長濾波器的微孔板閱讀器
? 高精度移液管和無菌移液管尖端
? Eppendorf管
? 37°C培養(yǎng)箱
? 去離子水或蒸餾水
? 吸水紙測試計(jì)劃
在使用該試劑盒之前，研究人員應(yīng)考慮以下事項(xiàng)：
1.完整閱讀本手冊(cè)，以盡量減少錯(cuò)誤
2.確認(rèn)您有合適的非供應(yīng)設(shè)備
3.確認(rèn)該試劑盒的種類、靶抗原和敏感性適合您的預(yù)期應(yīng)用。
4.確認(rèn)您的樣品已準(zhǔn)備妥當(dāng)根據(jù)建議（見樣品制備）足夠的樣本量用于分析。
5.首次使用試劑盒時(shí)，應(yīng)采取適當(dāng)?shù)尿?yàn)證步驟在使用有價(jià)值的樣品之前。確認(rèn)套件足夠通過以下方法檢測預(yù)期樣本類型中的目標(biāo)抗原運(yùn)行控制樣本。
6.如果樣本中的目標(biāo)抗原濃度為未知，應(yīng)使用控件運(yùn)行初步實(shí)驗(yàn)確定最佳樣品稀釋度的樣品（見實(shí)驗(yàn)布局和樣品制備）。
7.確保套件正確存放，不得超出其使用范圍使用到期日期。
8.當(dāng)使用同一試劑盒的多批次時(shí)，不要替換試劑從一個(gè)套件到另一個(gè)套件。在更改時(shí)仔細(xì)查看每個(gè)手冊(cè)可以在批次之間發(fā)生。控制分析間變異性包括遺留控制樣本。
WAVEFORM CAPTURE
The 369 stores 16 cycles of A/D samples each time a trip occurs in a trace buffer. The Trace Memory Trigger is set up in S1 Preferences and determines how many pre-trip and post-trip cycles are stored. The trace buffer is time and date stamped and may be correlated to a trip in the event record. 7 waveforms are captured this way when a trip occurs. These are the 3 phase currents, ground current and 3 voltage waveforms. The last three captured records are retained by the 369. This information is stored in volatile memory and will be lost if power is cycled to the relay. To access the captured waveforms, select the captured record by writing to the Trace Memory Buffer Selector (address 30F5h), then select waveform of interest by writing its trace memory channel (see following table) to the Trace Memory Channel Selector (address 30F6h). Then read the trace memory data from address 3100h to 31FFh. The values read are in actual amperes or volts.
KIT STORAGE
Upon receipt the kit should be stored at 4°C if intended for use within 24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B should be stored at -20°C. Avoid repeated freeze-thaw cycles. Store all other kit components at 4°C. The Substrate should never be frozen. Once individual reagents are opened it is recommended that the kit be used within 1 month. Unused Strip Plate wells should be stored at -20°C in a sealed bag containing desiccant in order to minimize exposure to moisture. Do not use the kit beyond its expiration date. OTHER REQUIRED SUPPLIES ? Microplate reader with 450nm wavelength filter ? High-precision pipette and sterile pipette tips ? Eppendorf tubes ? 37°C incubator ? Deionized or distilled water ? Absorbent paper
ASSAY PLANNING
Before using this kit, researchers should consider the following: 1. Read this manual in its entirety in order to minimize the chance of error. 2. Confirm that you have the appropriate non-supplied equipment available. 3. Confirm that the species, target antigen, and sensitivity of this kit are appropriate for your intended application. 4. Confirm that your samples have been prepared appropriately based upon recommendations (see Sample Preparation) and that you have sufficient sample volume for use in the assay. 5. When first using a kit, appropriate validation steps should be taken before using valuable samples. Confirm that the kit adequately detects the target antigen in your intended sample type(s) by running control samples. 6. If the concentration of target antigen within your samples is unknown, a preliminary experiment should be run using a control sample to determine the optimal sample dilution (see Experimental Layout and Sample Preparation). 7. Ensure that the kit is properly stored and do not use it beyond its expiration date. 8. When using multiple lots of the same kit do not substitute reagents from one kit to another. Review each manual carefully as changes can occur between lots. To control for inter-assay variability include a carry-over control sample.
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